Accelerated plaque formation by fowlpox virus in the presence of chymotrypsin.

Plaque formation of LaSota pathogenic strain of Newcastle disease virus adapted in chick embryo fibroblast cells

In order to adapt LaSota strain of Newcastle disease virus (NDV) on chick embryo fibroblast (CEF) cells, 0.1 ml from LaSota vaccine produced in Razi vaccine and serum research institute inoculated to the CEF cells and was passaged five times in the CEF grown in Eagle’s minimum essential medium (MEM). First to third passages were blind but in fourth passage cytopathic effect of virus was observe...

Reticuloendotheliosis virus sequences within the genomes of field strains of fowlpox virus display variability.

Nine field strains of fowlpox virus (FPV) isolated during a 24-year span from geographically diverse outbreaks of fowlpox in the United States were screened for the presence of reticuloendotheliosis virus (REV) sequences in their genomes by PCR. Each isolate appeared to be heterogeneous in that either a nearly intact provirus or just a 248- or 508-nucleotide fusion of portions of the integrated...

Human Immunodeficiency Virus (HIV) and Coronary Atherosclerotic Plaque Formation: A Review

Single amino acid change at the cleavage site of the fusion protein is responsible for both enhanced chymotrypsin sensitivity and trypsin resistance of a Sendai virus mutant, TR-5.

A trypsin-resistant mutant of Sendai virus, TR-5, which was obtained by passaging the wild-type (wt) virus in the presence of chymotrypsin followed by plaque purification, also had an enhanced susceptibility to activation by chymotrypsin. A trypsin-sensitive revertant, TSrev-58, derived from TR-5 had a decreased susceptibility to chymotrypsin and was susceptible to trypsin to the same degree as...

Chymotrypsin activity extracted from beluga (Huso huso) intestine under some physical and chemical factors

In the present study, the physical and chemical factors including optimum temperature and pH, the effect of inhibitors, metal ions, oxidizing agents, and surfactants were assayed on the chymotrypsin activity from beluga (Huso huso) intestine. According to the obtained results, the optimum temperature and pH of the chymotrypsin were recorded at 50°C and 8, respectively, and its activity was extr...